Coding

Part:BBa_K3136004:Design

Designed by: Liang ZHANG   Group: iGEM19_Shanghai_HS_United   (2019-09-18)


FnCas12a-typeIIS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 310
    Illegal PstI site found at 364
    Illegal PstI site found at 1627
    Illegal PstI site found at 2533
    Illegal PstI site found at 3727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 310
    Illegal PstI site found at 364
    Illegal PstI site found at 1627
    Illegal PstI site found at 2533
    Illegal PstI site found at 3727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1253
    Illegal BglII site found at 1586
    Illegal BglII site found at 1706
    Illegal BglII site found at 2000
    Illegal BglII site found at 2444
    Illegal BglII site found at 3335
    Illegal BglII site found at 3425
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 310
    Illegal PstI site found at 364
    Illegal PstI site found at 1627
    Illegal PstI site found at 2533
    Illegal PstI site found at 3727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 310
    Illegal PstI site found at 364
    Illegal PstI site found at 1627
    Illegal PstI site found at 2533
    Illegal PstI site found at 3727
    Illegal NgoMIV site found at 2480
    Illegal NgoMIV site found at 3319
    Illegal NgoMIV site found at 3530
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constucte the part as a coding sequence in typeIIS standard plasmid.


Source

It is a conding sequence of CRISPR-Cas12a from Francisella novicida U112. The sequence is coden optimized.

References